Whole cell immobilisation of Saccharomyces cerevisiae through attached growth process and its application in lipolytic enzyme production


Abstract:
Adaptation is the major utensil used by the microbial community to stay longer in evolution and one such feature is biofilm, wherein organisms attach on the surface of diverse origin through their secretion. Adhesion of micro-organism to the surface affords comfort in resistance against antibiotics, nutrient exchange and even genetic material to the closer microbial community. The study induces biofilm formation of Saccharomyces cerevisiae and optimising its attachment condition on different substrates for lipase production. S. cerevisiae was encouraged to grow on a solid matrix like calcium alginate beads and nylon beads in biofilm induction medium (BIM). Initial adhesion of yeast was screened on glass slide to evaluate its biofilm forming ability; surface immobilised beads were inoculated in YPD medium as repeated inoculum and its biomass was determined to check its stability of adhesion on these substrates. Further, these beads were inoculated in Tween 80 media for lipase production. To endorse the presence of biofilm, glass slides were stained with methylene blue to envision micro-organism; subsequently, immobilised nylon beads were autoclaved and inoculated into fresh YPD medium to authenticate microbial growth owing to the addition of immobilised yeast. Surface immobilisation of S. cerevisiae had shown increased biomass concentration in subsequent transfer. Promising results were obtained in nylon beads, and lipase enzymes were quantified. Surface immobilisation of micro-organism can be applied in fermentation industry to overcome the limits of entrapment methods since biofilm supports better adhesion over the surfaces and also these beads can be used for subsequent culturing in the production of enzymes.

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How to cite this article:
Lakshmi B, Chaitanya Lakshmi M, Rajesh Kumar U. Whole cell immobilisation of Saccharomyces cerevisiae through attached growth process and its application in lipolytic enzyme production. Journal of Biological and Information Sciences. 2012. 1 (3). 49-53.
Available from: http://ojournals.biolim.com/BOJ001/001/BOJ001A0012.